Journal: Food and Agricultural Immunology

Article Title: The effect of humic substances on gut microbiota and immune response of broilers

doi: 10.1080/09540105.2019.1707780

Figure Lengend Snippet: Figure 3. The effect of 0.8% of natural humic substances prepared from leonardite in feed on the per- centage of CD4+ CD8–, CD8+ CD4– and CD4+ CD8+ lymphocytes in broiler peripheral blood. Means with different superscripts are significantly different **p < .01.

Article Snippet: Tubes were centrifuged for 30 min at 600× g. MNL were recovered by aspirating white layer in plasma-LSM interphase, and the acquired sample was washed twice with PBS by centrifugation for 5 min at 250× g. Selected lymphocyte subpopulations were identified by direct staining with a subsequent combination of conjugated anti-chicken monoclonal antibodies (MoAb): CD4-FITC/ CD8a-R-PE/CD45-APC (Southern Biotech, USA).

Techniques:

Journal: Vaccine

Article Title: Construction and immunogenicity of a recombinant fowlpox vaccine coexpressing S1 glycoprotein of infectious bronchitis virus and chicken IL-18

doi: 10.1016/j.vaccine.2010.09.106

Figure Lengend Snippet: The percentage of CD3 + , CD4 + CD3 + and CD8 + CD3 + T-lymphocytes of different vaccine inoculated groups ( n = 4, i.e. number of times the test was repeated). Values are expressed as mean counts ± standard error. Statistically significant differences ( P < 0.05) are indicated by * (compared with S-FPV-017 or PBS) or ** (compared with rFPV-S1 alone).

Article Snippet: PBMC were adjusted to 1 × 10 7 cells/ml, and 100 μl of the resuspended cells (1 × 10 6 cells) were incubated for 1 h at room temperature with the following antibodies (double labeled): mouse anti-chicken CD3-Spectral Red (SPRD) and mouse anti-chicken CD4-R-phycoerythrin (R-PE) or mouse anti-chicken CD8a-R-PE (BD Biosciences Pharmingen).

Techniques:

Journal: Vaccine

Article Title: Construction and immunogenicity of a recombinant fowlpox vaccine coexpressing S1 glycoprotein of infectious bronchitis virus and chicken IL-18

doi: 10.1016/j.vaccine.2010.09.106

Figure Lengend Snippet: The percentage of CD3 + , CD4 + CD3 + and CD8 + CD3 + T-lymphocytes of different vaccine inoculated groups ( n = 4, i.e. number of times the test was repeated). Values are expressed as mean counts ± standard error. Statistically significant differences ( P < 0.05) are indicated by * (compared with S-FPV-017 or PBS) or ** (compared with rFPV-S1 alone).

Article Snippet: PBMC were adjusted to 1 × 10 7 cells/ml, and 100 μl of the resuspended cells (1 × 10 6 cells) were incubated for 1 h at room temperature with the following antibodies (double labeled): mouse anti-chicken CD3-Spectral Red (SPRD) and mouse anti-chicken CD4-R-phycoerythrin (R-PE) or mouse anti-chicken CD8a-R-PE (BD Biosciences Pharmingen).

Techniques:

Journal: Vaccine

Article Title: Construction and immunogenicity of a recombinant fowlpox vaccine coexpressing S1 glycoprotein of infectious bronchitis virus and chicken IL-18

doi: 10.1016/j.vaccine.2010.09.106

Figure Lengend Snippet: Ratio of CD4 + :CD8 + T-lymphocytes after vaccination. a

Article Snippet: PBMC were adjusted to 1 × 10 7 cells/ml, and 100 μl of the resuspended cells (1 × 10 6 cells) were incubated for 1 h at room temperature with the following antibodies (double labeled): mouse anti-chicken CD3-Spectral Red (SPRD) and mouse anti-chicken CD4-R-phycoerythrin (R-PE) or mouse anti-chicken CD8a-R-PE (BD Biosciences Pharmingen).

Techniques: